Sunday, March 6, 2011

How do I figure out these Dilutions?

DNA marker usually comes at a concentration of 1 mg/ml. I like to dilute it so that when I load 6 ul of marker on a gel, I end up loading 1ug. If you start with 250 ul of 1 mg/ml DNA ladder, how much water, 6x orange G loading dye, and 6x bromophenol blue loading dye would you have to add so that the final concentration of DNA is ul/ml and the final concentrations of each dye are 1x?

No comments:

Post a Comment